FGF1 Protein, Canine, Recombinant

• Background:

  AFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

• Gene Name:

  Fibroblast growth factor 1 (acidic)

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 90 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts.The EDfor this effect is typically 0.05-0.3 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Diagnostic proteins, Growth Factor proteins, Cytokine Proteins, Cell Therapy proteins, Biological Target proteins, Biomarker Proteins, Enzyme Proteins

• Molecular Weight:

  The recombinant canine FGF1 comprises 141 amino acids and has a predicted molecular mass of 16 kDa. The apparent molecular mass of the protein is approximately 16 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FGF1

• Species:

  Canine

• Protein ID:

  XP_849274.1

• Overview:

  A DNA sequence encoding the canine FGF1 (XP_849274.1) (Phe16-Asp155) was expressed with a N-terminal Met.

• AA Sequence:

  Phe16-Asp155

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/XP_849274.1