FGF1 (NM_000800) Human Recombinant Protein

• Background:

  The protein encoded by this gene is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Multiple alternatively spliced variants encoding different isoforms have been described. [provided by RefSeq, Jan 2009]

• Synonyms:

  AFGF; ECGF; ECGF-beta; ECGFA; ECGFB; FGF-1; FGF-alpha; FGFA; GLIO703; HBGF-1; HBGF1

• Gene ID:

  2246

• UniProt:

  P05230

• Accession Number mRNA:

  NM_000800

• Chromosomal Location:

  5q31.3

• Expression System:

  E. coli

• Tag:

  Tag Free

• Related Pathways:

  MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

• Field of Research:

  MAPK signaling pathway, Melanoma, Pathways in cancer, Regulation of actin cytoskeleton

• Concentration:

  Resuspend the protein to the desired concentration in proper buffer.

• Endotoxin:

  < 0.1 EU per 1 µg of the protein by the LAL

• Purity:

  >95% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  Measured in a cell proliferation assay using 3T3 cells. ED50 for this effect is typically 0.031 - 0.046 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from a sterile solution containing 50 mM Tris.Cl, pH7.5, 150mM NaCl.

• Function:

  Druggable Genome, Secreted Protein

• Molecular Weight:

  15.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FGF1

• Species:

  Human

• Protein ID:

  NP_000791

• Overview:

  Recombinant protein of human Fibroblast Growth Factor-acidic (FGF1) produced in E. coli

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/2246

• Uniprot URL:

  https://www.uniprot.org/uniprot/P05230

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_000800

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_000791