FGF1 Protein, Human, Recombinant

• Background:

  AFGF, also known as FGF1 and HBGF-1, is a member of the fibroblast growth factor family. The biological activity of aFGF protein is exerted through binding to four high affinity cell surface receptors (FGFR1–4), which results in receptor dimerization and transphosphorylation in the tyrosine kinase domain. aFGF protein shows a wide range of endocrine-like activities. As a multiple function growth factor, this protein is involved in embryo development and tissue repair. Additionally, this protein is considered to function in several important physiological and pathological processes, such as embryonic development, morphogenesis, angiogenesis, wound healing and atheromatosis, carcinogenesis, development, and invasion of cancer.

• Synonyms:

  AFGF Protein, Human; ECGF Protein, Human; ECGF-beta Protein, Human; ECGFA Protein, Human; ECGFB Protein, Human; FGF-1 Protein, Human; FGF-alpha Protein, Human; FGFA Protein, Human; GLIO703 Protein, Human; HBGF-1 Protein, Human; HBGF1 Protein, Human

• Gene Name:

  Fibroblast growth factor 1 (acidic)

• Expression System:

  E. coli

• Tag:

  No tag

• Endotoxin:

  Please contact us for more information.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The EDfor this effect is typically 0.15-0.75 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Biological Target proteins, Diagnostic proteins, Growth Factor proteins, Biomarker Proteins, Enzyme Proteins, Cell Therapy proteins

• Molecular Weight:

  The recombinant human FGF acidic consists of 141 amino acids and has a calculated molecular mass of 16 kDa as estimated by SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FGF1

• Species:

  Human

• Protein ID:

  AAA79245.1

• Overview:

  A DNA sequence encoding the mature form of human FGF acidic (AAA79245.1) (Phe 16-Asp 155) was expressed, with an additional Met at the N-terminus.

• AA Sequence:

  Phe16-Asp155

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/AAA79245.1