CD32B/Fcgr2b Protein, Cynomolgus, Recombinant (His & AVI Tag), Biotinylated

• Gene Name:

  Fc fragment of IgG, low affinity IIb, receptor (CD32)

• Expression System:

  HEK293 Cells

• Tag:

  C-AVI & His

• Field of Research:

  Fc Receptors ITIM/ITAM Immunoreceptors and Related Molecules

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 97 % as determined by SDS-PAGE.

• Activity:

  Immobilized Cynomolgus CD32B/Fcgr2b Protein (His & AVI Tag), Biotinylated (Cat:PK53968M9) at 2 μg/mL (100 μL/well) can bind Human IgG2 Protein (257 Ser/Ala) (Cat:13504-HNAH), the ECis 4-36 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Fc receptor proteins, Immunotherapy proteins, Biotinylated proteins, CD Molecule proteins, Receptor Proteins

• Molecular Weight:

  The recombinant cynomolgus CD32b consists of 211 amino acids and has a calculated molecular mass of 23.5 kDa. The apparent molecular mass of the recombinant protein is approximately 34 kDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  FCGR2B

• Species:

  Cynomolgus

• Protein ID:

  AAL92097.1

• Overview:

  A DNA sequence encoding the Cynomolgus (Macaca fascicularis) CD32b (AAL92097.1) extracellular domain (Met 1-Gly 220) was fused with a c-terminal polyhistidine tagged AVI tag at the C-terminus. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed.

• AA Sequence:

  Met1-Gly220

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/AAL92097.1