Galectin-1 Protein, Mouse, Recombinant

Galectin-1 (Gal-1, GAL1), is a member of the galectins, a family of animal lectins ranging from Caenorhabditis elegans to humans, which is defined by their affinity for beta-galactosides and by significant sequence similarity in the carbohydrate-binding site. It is a homodimer with a subunit molecular mass of 14.5 kDa, which contains six cysteine residues per subunit. The cysteine residues should be in a free state to maintain a molecular structure that is capable of showing lectin activity. This endogenous lectin widely expressed at sites of inflammation and tumor growth has been postulated as an attractive immunosuppressive agent to restore immune cell tolerance and homeostasis in autoimmune and inflammatory settings. On the other hand, galectin-1 contributes to different steps of tumor progression including cell adhesion, migration, and tumor-immune escape, suggesting that blockade of galectin-1 might result in therapeutic benefits in cancer. Several potential glycoprotein ligands for galectin-1 have been identified, including lysosome-associated membrane glycoproteins and fibronectin, laminin, as well as T-cell glycoproteins CD43 and CD45. Evidence points to Gal-1 and its ligands as one of the master regulators of such immune responses as T-cell homeostasis and survival, T-cell immune disorders, inflammation, and allergies as well as host-pathogen interactions.

A DNA sequence encoding the mouse Lgals1 (P16045) (Met 1-Glu 135) was expressed and purified.

AA410090 Protein, Mouse; Gal-1 Protein, Mouse; Galbp Protein, Mouse; galectin-1 Protein, Mouse; L-14.5 Protein, Mouse; L14 Protein, Mouse; Lect14 Protein, Mouse

Lectin, galactoside-binding, soluble, 1

P16045

E. coli

No tag

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> 95 % as determined by SDS-PAGE.

Measured by its ability to agglutinate human red blood cells. The EDfor this effect is typically 6-60 μg/ml.

Lyophilized

Lyophilized from sterile PBS, pH7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Cell Therapy proteins

The recombinant mouse Lgals1 consists of 135 amino acids and migrates as an approximately 15 kDa band as predicted in SDS-PAGE under reducing conditions.

In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

LGALS1

Mouse

https://www.uniprot.org/uniprot/P16045