ICAM-2 Protein, Mouse, Recombinant (hFc Tag)

• Background:

  Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18) . As the second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocyte with other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity, and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relationship with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in the diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.

• Synonyms:

  CD102 Protein, Mouse; Icam-2 Protein, Mouse; Ly-60 Protein, Mouse

• Gene Name:

  Intercellular adhesion molecule 2

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 93 % as determined by SDS-PAGE.

• Activity:

  Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When cells are added to mouse ICAM2 coated plates (12.5 μg/ml, 100 μl/well), approximately 25-40% will adhere specifically.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 50mM Tris-Citric acid, 150mM NaCl, pH 5.5 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  CD Molecule proteins

• Molecular Weight:

  The secreted recombinant mouse ICAM2/Fc is a disulfide-linked homodimeric protein. The reduced monomer comprises 444 amino acids and has a predicted molecular mass of 50 kDa. As a result of glycosylation, the apparent molecular mass of rm ICAM2/Fc monomer is approximately 60-70 kDa in SDS-PAGE under reducing conditions.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  ICAM2

• Species:

  Mouse

• Protein ID:

  NP_034624.1

• Overview:

  A DNA sequence encoding the mouse ICAM2 (NP_034624.1) extracellular domain (Met 1-Gln 222) was fused with the Fc region of human IgG1 at the C-terminus.

• AA Sequence:

  Met1-Gln222

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_034624.1