Recombinant Mouse ICAM-2/CD102 Protein

Recombinant Mouse ICAM-2/CD102 Protein

• Background: Intercellular adhesion molecule 2 (ICAM-2, CD102), belongs to the ICAM family consisting of three members identified as ligands for integrin receptors. It is a type I transmembrane glycoprotein with two Ig-like C2-type domains, and binds to the leukocyte integrins LFA-1 (CD11a/CD18) and Mac-1 (CD11b/CD18). As a second ligand of leukocyte function-associated antigen-1, ICAM-2 functions as a costimulatory molecule for effector cells. ICAM-2 is mainly expressed on vascular endothelial and hematopoietic cells. Interactions of ICAM-2 and the integrin receptors mediate cell adhesion in a wide range of lymphocyte, monocyte, natural killer cell, and granulocytewith other cells, and play important roles in many adhesion-dependent immune and inflammation responses, such as T cell aggregation, NK-cell cytotoxicity and migration, lymphocyte recirculation, etc. Serum levels of ICAM-2 correlated significantly with the inflammatory and course sequences of trichinosis in mice and had a similar relation with blood eosinophilia. So, estimation of ICAM-2 serum levels may prove useful in diagnosis of trichinosis recent infections, and in monitoring the prognosis and response to treatment.
• Synonyms: AILIM Protein, CCLP Protein, CRP-1 Protein, H4 Protein, Ly115 Protein
• CAS Number: 9000-83-3
• UniProt: P35330
• Accession Number: NP_034624.1
• Host: HEK293 Cells
• Origin Species: Mouse
• Tag: None
• Sequence: Met 1-Gln 222
• Applications: Cell culture
• Purity: > 85 % as determined by reducing SDS-PAGE.
• Bioactivity: Measured by the ability of the immobilized protein to support the adhesion of PMA-stimulated HSB2 human peripheral blood acute lymphoblastic leukemia cells. When 5 x 10⁴ cells/well are added to rmICAM1 coated plates (12.5 μg/mL with 100 μL/well), > 30% cells will adhere after 1 hour incubation at 37°C.
• Reconstitution: Please refer to the printed manual for detailed information.
• Molecular Weight: 38-42 kDa
• Shipping Conditions: This product is provided as lyophilized powder which is shipped with ice packs.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.