Lif (NM_008501) Mouse Recombinant Protein

• Background:

  LIF has the capacity to induce terminal differentiation in leukemic cells. Its activities include the induction of hematopoietic differentiation in normal and myeloid leukemia cells, the induction of neuronal cell differentiation, and the stimulation of acute-phase protein synthesis in hepatocytes.[UniProtKB/Swiss-Prot Function]

• Gene ID:

  16878

• UniProt:

  P09056

• Accession Number mRNA:

  NM_008501

• Chromosomal Location:

  11 2.94 cM

• Expression System:

  E. coli

• Tag:

  Tag Free

• Concentration:

  Resuspend the protein to the desired concentration in proper buffer.

• Endotoxin:

  < 0.1 EU per 1 µg of the protein by the LAL

• Purity:

  >95% as determined by SDS-PAGE and Coomassie blue staining

• Activity:

  Measured by its ability to induce differentiation of M1 cells. ED50 for this effect is typically 0.20-0.30 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from a sterile solution containing 20 mM phosphate buffer, pH7.4.

• Molecular Weight:

  19.9 kDa

• Storage Conditions:

  Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  Lif

• Species:

  Mouse

• Protein ID:

  NP_032527

• Overview:

  Recombinant protein of Mus musculus leukemia inhibitory factor (Lif) produced in E. coli.

• Gene ID URL:

  https://www.ncbi.nlm.nih.gov/gene/16878

• Uniprot URL:

  https://www.uniprot.org/uniprot/P09056

• Accession Number mRNA URL:

  https://www.ncbi.nlm.nih.gov/nuccore/NM_008501

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/nuccore/NP_032527