Products for Research Use Only
RegisterLogin

BACE1 Protein, Human, Recombinant

CAT: 0882-PH52014M0Size: 100 µgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0882-PH52014M0Size:100 µg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Background
Beta-site APP-cleaving enzyme 1 (BACE1) is an aspartic-acid protease important in the formation of myelin sheaths in peripheral nerve cells. In the brain, This protein is expressed highly in the substantia nigra, locus coruleus and medulla oblongata. Strong BACE1 expression has also been described in pancreatic tissue. BACE1 has a pivotal role in the pathogenesis of Alzheimer's disease. In Alzheimer's disease patients, BACE1 levels were elevated although mRNA levels were not changed. It has been found that BACE1 gene expression is controlled by a TATA-less promoter. The translational repression as a new mechanism controlling its expression. And the low concentrations of Ca (2+) (microM range) significantly increased the proteolytic activity of BACE1. Furthermore, BACE1 protein is ubiquitinated, and the degradation of BACE1 proteins and amyloid precursor protein processing are regulated by the ubiquitin-proteasome pathway. It has also been identified as the rate limiting enzyme for amyloid-beta-peptide (Abeta) production.
Overview
The mature form of human BACE1 (NP_036236.1) extracellular domain (Met1-Thr 457) with a quinary-aa peptide (DDDDK) at the C-terminus was expressed and purified.
Synonyms
ASP2 Protein, Human; BACE Protein, Human; HSPC104 Protein, Human
Gene Name
Beta-site APP-cleaving enzyme 1
Expression System
HEK293 Cells
Tag
No tag
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 95 % as determined by SDS-PAGE.
Activity
Measured by its ability to cleave a fluorescent peptide substrate Mca-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Arg-Lys (Dpn) -Arg-Arg-NH2 (Catalog# ES004, R&D Systems) . Cleavage of ES004 can be measured using excitation and emission wavelength of 320 and 405 nm, respectively.The specific activity is >3.5 pmoles/min/μg.
Form
Lyophilized
Buffer
Lyophilized from sterile 50mM Tris, 100mM NaCl, pH 8.0 Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Function
Enzyme Proteins
Molecular Weight
The recombinant human BACE1 consists of 442 amino acids and has a calculated molecular mass of 49 kDa. As a result of glycosylation, the rhBACE1 migrates as an approximately 65 kDa protein in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
BACE1
Species
Human
Protein ID
NP_036236.1
AA Sequence
Met1-Thr457
Protein ID Link
https://www.ncbi.nlm.nih.gov/protein/NP_036236.1

Related Products

CatalogName
PH37797M5BACE1 (NM_012104) Human Recombinant Protein
PM45989M5Bace1 (NM_011792) Mouse Recombinant Protein
PM52855M2-01BACE1 Protein, Mouse, Recombinant (His Tag)
APR11484GPolyclonal BACE1 / BACE Antibody (Internal)
AMM05613GPolyclonal BACE1 / BACE Antibody (C-Terminus)
APG03188GPolyclonal BACE1 Antibody
LH01244VBACE1 (NM_138971) Human Over-expression Lysate
LH18317VBACE1 (NM_012104) Human Over-expression Lysate
LH13907VBACE1 (NM_138973) Human Over-expression Lysate
LH13908VBACE1 (NM_138971) Human Over-expression Lysate

Alternative Products

CatalogName
TMPY-00652-01BACE1 Protein, Human, Recombinant
MBS1560996-01Recombinant Human BACE1 protein
RP002572BACE1 Recombinant Protein (Human)
PPT-10788Human BACE1 Recombinant Protein
96-051BACE1 Recombinant Protein
MBS554257-01Human BACE1 Recombinant
MBS7608633-01Recombinant Human BACE1
ARP7258-01Recombinant Human BACE1 / ASP2 Protein
TP309115MBACE1 (NM_012104) Human Recombinant Protein
BACE1-255HActive Recombinant Human BACE1 protein

Popular Products