M-CSF Protein, Human, Recombinant

• Background:

  Macrophage colony-stimulating factor 1, also known as CSF-1, M-CSF, Lanimostim and CSF1, is a single-pass membrane protein which is disulfide-linked as a homodimer or heterodimer. Granulocyte / macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. M-CSF/CSF-1 is known to facilitate monocyte survival, monocyte-to-macrophage conversion, and macrophage proliferation. M-CSF/CSF-1 is a secreted cytokine which influences hemopoietic stem cells to differentiate into macrophages or other related cell types. It binds to the Colony stimulating factor 1 receptor. M-CSF/CSF-1 may also be involved in development of the placenta. The active form of M-CSF/CSF-1 is found extracellularly as a disulfide-linked homodimer, and is thought to be produced by proteolytic cleavage of membrane-bound precursors. M-CSF/CSF-1 induces cells of the monocyte/macrophage lineage. It also plays a role in immunological defenses, bone metabolism, lipoproteins clearance, fertility and pregnancy. Upregulation of M-CSF/CSF-1 in the infarcted myocardium may have an active role in healing not only through its effects on cells of monocyte/macrophage lineage, but also by regulating endothelial cell chemokine expression.

• Synonyms:

  CSF-1 Protein, Human; M-CSF Protein, Human; MCSF Protein, Human

• Gene Name:

  Colony stimulating factor 1 (macrophage)

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  Measured in a cell proliferation assay using M NFS 60 mouse myelogenous leukemia lymphoblast cells.The EDfor this effect is typically 1.6-6.3 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Cytokine Proteins, Growth Factor proteins, Biological Target proteins, Diagnostic proteins, Biomarker Proteins

• Molecular Weight:

  The recombinant human CSF1 consists 158 amino acids and predicts a molecular mass of 18.4 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CSF1

• Species:

  Human

• Protein ID:

  NP_757349.1

• Overview:

  A DNA sequence encoding the human CSF1 (NP_757349.1) (Met1-Asn190) was expressed with an initial Met.

• AA Sequence:

  Met1-Asn190

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_757349.1