P67phox Protein, Human, Recombinant

• Background:

  NCF2 (Neutrophil Cytosolic Factor 2, also known as NCF-2 and p67phox) is a Protein Coding gene. 4 alternatively spliced human isoforms have been reported. This gene encodes neutrophil cytosolic factor 2, the 67-kilodalton cytosolic subunit of the multi-protein NADPH oxidase complex found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. NCF2 belongs to the NCF2/NOXA1 family. NCF2, NCF1, and a membrane-bound cytochrome b558 are required for activation of the latent NADPH oxidase. Mutations in the NCF2 gene, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease, a disease that causes recurrent infections by catalase-positive organisms.

• Synonyms:

  NCF-2 Protein, Human; NOXA2 Protein, Human; P67-PHOX Protein, Human; P67PHOX Protein, Human

• Gene Name:

  Neutrophil cytosolic factor 2

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 300mM NaCl, pH 8.0. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The secreted recombinant human NCF2 consists of 528 amino acids and predicts a molecular mass of 59.9 KDa. The apparent molecular mass of the protein is approximately 60 KDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  NCF2

• Species:

  Human

• Protein ID:

  AAH01606.1

• Overview:

  A DNA sequence encoding the human NCF2 (AAH01606.1) (Met1-Val526) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

• AA Sequence:

  Met1-Val526

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/AAH01606.1