GCAP1 Protein, Human, Recombinant

• Background:

  GCAP 1 gene plays a role in the recovery of retinal photoreceptors from photobleaching. In the recovery phase, the phototransduction messenger cGMP is replenished by retinal guanylyl cyclase-1 (GC1) . GC1 is activated by decreasing Ca (2+) concentrations following photobleaching. The protein encoded by this gene, guanylyl cyclase-activating protein 1 (GCAP 1), mediates the sensitivity of GC1 to Ca (2+) concentrations. GCAP 1 promotes the activity of GC1 at low Ca (2+) concentrations and inhibits GC1 activity at high Ca (2+) concentrations. Mutations in GCAP 1 gene cause autosomal dominant cone dystrophy (COD3) ; a disease characterized by reduced visual acuity associated with progressive loss of color vision. GCAP 1 stimulates guanylyl cyclase 1 (GC1) when free calcium ions concentration is low and inhibits GC1 when free calcium ions concentration is elevated. This Ca (2+) -sensitive regulation of GC is a key event in the recovery of the dark state of rod photoreceptors following light exposure.

• Synonyms:

  C6orf131 Protein, Human; COD3 Protein, Human; CORD14 Protein, Human; dJ139D8.6 Protein, Human; GCAP Protein, Human; GCAP1 Protein, Human; GUCA Protein, Human; GUCA1 Protein, Human

• Gene Name:

  Guanylate cyclase activator 1A (retina)

• UniProt:

  P43080

• Expression System:

  Baculovirus-Insect Cells

• Tag:

  No tag

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 85 % as determined by SDS-PAGE.

• Activity:

  At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile 20mM Tris, 500mM NaCl, 10% glycerol, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Molecular Weight:

  The secreted recombinant human GUCA1A consists of 203 amino acids and predicts a molecular mass of 23.1 KDa. The apparent molecular mass of the protein is approximately 20 KDa in SDS-PAGE under reducing conditions due to glycosylation.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  GUCA1A

• Species:

  Human

• Overview:

  A DNA sequence encoding the human GUCA1A (P43080) (Met1-Gly201) was expressed and purified with two additional amino acids (Gly & Pro) at the N-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P43080

• AA Sequence:

  Met1-Gly201