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RAB27B Protein, Human, Recombinant

CAT: 0882-PH50330M0Size: 100 µgDry Ice: NoHazardous: No
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CAT#:0882-PH50330M0Size:100 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
RAB27B is a member of the Rab family GTPases involved in vesicle trafficking. The Rab27 subfamily consists of Rab27a and Rab27b. The human RAB27B gene is organized in six exons, spanning about 69 kb in the chromosome 18q21.1 region. The Rab27 subfamily of Ras-like GTPases is highly conserved in mammals. There is high degree of conservation in sequence and gene structure between RAB27A and RAB27B genes. Exogenous expression of Rab27b in melanocytes results in melanosomal association as observed for Rab27a, suggesting the two Rab27 proteins are functional homologues. As with RAB27A in Griscelli Disease, RAB27B may be also associated with human disease mapping to chromosome 18.
Overview
A DNA sequence encoding the human RAB27B (O00194) (Thr2-Lys215) was expressed with two additional amino acids (Gly & Pro) at the N-terminus.
Synonyms
C25KG Protein, Human; RAB27B Protein, Human
Gene Name
RAB27B, member RAS oncogene family
UniProt
O00194
Expression System
HEK293 Cells
Tag
No tag
Endotoxin
< 1.0 EU per μg of the protein as determined by the LAL method.
Purity
> 85 % as determined by SDS-PAGE.
Activity
At Leading Biology, the biological activity of a recombinant protein is routinely measured using a bioassay, e.g. chemotaxis or cell proliferation assay, enzyme assay, or a functional ELISA. When a recombinant protein is an enzyme, specific activity is derived from an enzymatic assay. Each enzyme is tested for potency by cleavage of a substrate. We are in the process of updating the biological activity data. If you have any questions regarding this update, please feel free to contact our technical support team.
Form
Lyophilized
Buffer
Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.
Molecular Weight
The recombinant human RAB27B comprises 216 amino acids and has a predicted molecular mass of 24.3 kDa. The apparent molecular mass of the monomer is approximately 27 kDa in SDS-PAGE under reducing conditions.
Storage Conditions
In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.
Symbol
RAB27B
Species
Human
Uniprot URL
https://www.uniprot.org/uniprot/O00194
AA Sequence
Thr2-Lys215

UniProtKB · O00194

Ras-related protein Rab-27B

RB27B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
O00194
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
RAB27B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.6.5.2
Processing
—
Secondary accessions
B2RAB0, Q9BZB6
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationDisulfide bondLipoproteinMethylationPrenylation

Cellular component

EndosomeMembrane

Ligand

GTP-bindingMagnesiumMetal-bindingNucleotide-binding

Molecular function

Hydrolase

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