CTLA-4 Protein, Human, Recombinant

• Background:

  Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2) . CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg) .

• Synonyms:

  ALPS5 Protein, Human; CD Protein, Human; CD152 Protein, Human; CELIAC3 Protein, Human; CTLA-4 Protein, Human; GRD4 Protein, Human; GSE Protein, Human; IDDM12 Protein, Human

• Gene Name:

  Cytotoxic T-lymphocyte-associated protein 4

• Expression System:

  HEK293 Cells

• Tag:

  No tag

• Field of Research:

  ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA. Immobilized CTLA-4 Protein, Human, Recombinant (Cat:PH50268M0) at 2 μg/mL (100 μL/well) can bind B7-1 Protein, Human, Recombinant (His & hFc Tag), Biotinylated, the ECof B7-1 is 6-27 ng/mL.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  B7 Family proteins, CD Molecule proteins, Cancer proteins, Receptor Proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

• Molecular Weight:

  The recombinant human CTLA4 consists of 126 amino acids and predicts a molecular mass of 13.5 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CTLA4

• Species:

  Human

• Protein ID:

  NP_005205.2

• Overview:

  A DNA sequence encoding the human CTLA4 (NP_005205.2) (Met1-Asp161) was expressed.

• AA Sequence:

  Met1-Asp161

• Protein ID Link:

  https://www.ncbi.nlm.nih.gov/protein/NP_005205.2