CTLA-4 Protein, Cynomolgus, Rhesus, Recombinant (hFc Tag)

• Background:

  Cytotoxic T-lymphocyte protein 4, also known as CTLA4 and CD152, is a single-pass type I membrane protein and a member of the immunoglobulin superfamily. It is the second member of the CD28 receptor family. The ligands or counterreceptors for these two proteins are the B7 family members, CD80 (B7-1) and CD86 (B7-2) . CTLA4 transmits an inhibitory signal to T cells, whereas CD28 transmits a stimulatory signal. Intracellular CTLA4 is also found in regulatory T cells and may play an important role in their functions. CD152 or cytotoxic T lymphocyte antigen-4 (CTLA-4) is an essential receptor involved in the negative regulation of T cell activation. Because of its profound inhibitory role, CD152 has been considered a sound susceptible candidate in autoimmunity and a persuasive target for cancer immunotherapy. In particular, recent evidence suggests that CD152 is also important in the homeostasis and function of a population of suppressive cells, termed regulatory T cells (Treg) .

• Gene Name:

  Cytotoxic T-lymphocyte-associated protein 4

• UniProt:

  Q9BDC4

• Expression System:

  HEK293 Cells

• Tag:

  C-hFc

• Field of Research:

  ITIM/ITAM Immunoreceptors and Related Molecules Cancer Drug Targets

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its binding ability in a functional ELISA.Immobilized human B7-1-His (Cat:PH51938M2) at 10 μg/ml (100 μl/well) can bind Cynomolgus CTLA4-Fc (Cat:PK54111M4), The ECof Cynomolgus CTLA4-Fc is 6.0-14.5 ng/ml.

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  B7 Family proteins, CD Molecule proteins, Receptor Proteins, Cancer proteins, Immunotherapy proteins, Immune Checkpoint Proteins, Biological Target proteins

• Molecular Weight:

  The recombinant cynomolgus / rhesus CTLA4 is a disulfide-linked homodimer. The reduced monomer comprises 366 amino acids and has a calculated molecular mass of 40.4 KDa.The apparent molecular mass of the protein is approximately 52 KDa respectively in SDS-PAGE.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  CTLA4

• Species:

  Cynomolgus, Rhesus

• Overview:

  A DNA sequence encoding the cynomolgus / rhesus CTLA4 (Q9BDC4) (Met1-Asp161) was expressed with the Fc region of human IgG1 at the C-terminus. Cynomolgus and Rhesus CTLA4 sequences are identical.

• Uniprot URL:

  https://www.uniprot.org/uniprot/Q9BDC4

• AA Sequence:

  Met1-Asp161