Prostate Specific Antigen/KLK3 Protein, Human, Recombinant (His Tag)

• Background:

  KLK3 (Kallikrein Related Peptidase 3) is a Protein Coding gene. The gene is one of the fifteen kallikrein subfamily members located in a cluster on chromosome 19. It encodes a single-chain glycoprotein, a protease that is synthesized in the epithelial cells of the prostate gland and is present in seminal plasma. KLK3, also known as Prostate Specific Antigen (PSA), kallikrein-related peptidase 3, Gamma-seminoprotein, is a secreted protein of the glandular kallikrein subfamily of serine proteases. KLK3 contains one peptidase S1 domain. KLK3 is a glycoprotein produced almost exclusively by the prostate gland. Growing evidence suggests that many kallikreins are implicated in carcinogenesis and some have potential as novel cancer and other disease biomarkers.

• Synonyms:

  APS Protein, Human; Hk3 Protein, Human; KLK2A1 Protein, Human; PSA Protein, Human

• Gene Name:

  Kallikrein related peptidase 3

• UniProt:

  P07288

• Expression System:

  HEK293 Cells

• Tag:

  C-His

• Endotoxin:

  < 1.0 EU per μg of the protein as determined by the LAL method.

• Purity:

  > 95 % as determined by SDS-PAGE.

• Activity:

  Measured by its ability to cleave the colorimetric peptide substrate, Succinyl-Arg-Pro-Tyr-p-Nitroanilide (Suc-RPY-pNA) . The specific activity is >100 pmoles/min/μg. (Activation description: The proenzyme needs to be activated by Thermolysin for an activated form)

• Form:

  Lyophilized

• Buffer:

  Lyophilized from sterile PBS, pH 7.4. Please contact us for any concerns or special requirements. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA.

• Function:

  Immunotherapy proteins, Biomarker Proteins, Enzyme Proteins, Protease proteins, Diagnostic proteins

• Molecular Weight:

  The recombinant human KLK3 consists of 255 amino acids and has a predicted molecular mass of 28.3 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rh KLK3 is approximately 35 kDa.

• Storage Conditions:

  In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature. Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise. Stable for 1 year at -20°C or below from date of shipment. For maximum recovery of product, centrifuge the original vial after thawing and opening the cap. For long-term storage, aliquot and store at -20°C or below. Avoid repeated freeze-thaw cycles.

• Symbol:

  KLK3

• Species:

  Human

• Overview:

  A DNA sequence encoding the human KLK3 (P07288) (Met 1-Pro 261) was fused with a polyhistidine tag at the C-terminus.

• Uniprot URL:

  https://www.uniprot.org/uniprot/P07288

• AA Sequence:

  Met1-Pro261