Anti-Biotin HRP

• Certification:

  RUO

• Immunogen:

  Biotinylated limulus hemocyanin

• Target Antigen:

  Biotin

• Clone:

  1D4-C5

• Applications:

  ELISA (QC tested)

• Concentration:

  0.2 mg/mL

• Format:

  HRP

• Buffer:

  Stabilizing phosphate buffered saline (PBS), pH 7.4, 0.01% thimerosal

• References & Citations:

  *Green NM, Moody KS, Debatis M, Marshak-Rothstein A: Activation of autoreactive B cells by endogenous TLR7 and TLR3 RNA ligands. J Biol Chem. 2012 Nov 16;287 (47) :39789-99. , URL: https://pubmed.ncbi.nlm.nih.gov/23019335/, *Uccellini MB, Busconi L, Green NM, Busto P, Christensen SR, Shlomchik MJ, Marshak-Rothstein A, Viglianti GA: Autoreactive B cells discriminate CpG-rich and CpG-poor DNA and this response is modulated by IFN-alpha. J Immunol. 2008 Nov 1;181 (9) :5875-84., URL: https://pubmed.ncbi.nlm.nih.gov/18941176/, *Stensland ZC, Smith MJ: Enrichment and detection of antigen-binding B cells for mass cytometry. Magnetochemistry. 2021 Jul;7 (7) :92., URL: https://pubmed.ncbi.nlm.nih.gov/34295938/

• Storage Conditions:

  Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

• Specificity:

  Mouse monoclonal antibody 1D4-C5 recognizes biotin and can be used as a second-step reagent for detecting biotinylated antigens.

• Isotype:

  Mouse IgG2a kappa