Anti-TRIM Biotin

• Description:

  TRIM (T cell receptor-interacting molecule), also known as TRAT1 (T cell receptor associated transmembrane adaptor 1) is a 30 kDa protein expressed by T cells as a cystein-linked homodimer. It associates with TCR-CD3-zeta-chain complex and becomes phosphorylated by Src-family kinases. TRIM is potentially involved in negative regulation of TCR-mediated signaling, but its role remains unclear.

• Certification:

  RUO

• Negative Species:

  Mouse

• Immunogen:

  Recombinant intracellular domain (aa 29-186) of human TRIM.

• Target Antigen:

  TRIM

• Clone:

  TRIM-04

• Applications:

  IP, FC-IC, WB (QC tested)

• Concentration:

  1 mg/mL

• Format:

  Biotin

• Buffer:

  Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

• References & Citations:

  *Kirchgessner H, Dietrich J, Scherer J, Isomaki P, Korinek V, Hilgert I, Bruyns E, Leo A, Cope AP, Schraven B.: The transmembrane adaptor protein TRIM regulates T cell receptor (TCR) expression and TCR-mediated signaling via an association with the TCR zeta chain. J Exp Med. 2001 Jun 4;193 (11) :1269-84. , URL: https://www.ncbi.nlm.nih.gov/pubmed/11390434?dopt=

• Other References:

  *Tedoldi S, Paterson JC, Hansmann ML, Natkunam Y, Rüdiger T, Angelisova P, Du MQ, Roberton H, Roncador G, Sanchez L, Pozzobon M, Masir N, Barry R, Pileri S, Mason DY, Marafioti T, Horejsí V: Transmembrane adaptor molecules: a new category of lymphoid-cell markers. Blood. 2006 Jan 1;107 (1) :213-21., URL: https://pubmed.ncbi.nlm.nih.gov/16160011/, *Simeoni L, Smida M, Posevitz V, Schraven B, Lindquist JA: Right time, right place: the organization of membrane proximal signaling. Semin Immunol. 2005 Feb;17 (1) :35-49., URL: https://pubmed.ncbi.nlm.nih.gov/15582487/, *Huynh T, Wurch A, Bruyns E, Korinek V, Schraven B, Eichmann K: Developmentally regulated expression of the transmembrane adaptor protein trim in fetal and adult T cells. Scand J Immunol. 2001 Jul-Aug;54 (1-2) :146-54., URL: https://www.ncbi.nlm.nih.gov/pubmed/11439161?dopt=, *Horejsí V, Zhang W, Schraven B: Transmembrane adaptor proteins: organizers of immunoreceptor signalling. Nat Rev Immunol. 2004 Aug;4 (8) :603-16., URL: https://pubmed.ncbi.nlm.nih.gov/15286727/

• Storage Conditions:

  Store at 2-8°C. Do not freeze.

• Specificity:

  The antibody TRIM-04 recognizes an intracellular epitope of T cell receptor-interacting molecule (TRIM), a 30 kDa adaptor protein expressed by T cells.

• Species:

  Human

• Isotype:

  Mouse IgG2a