Products for Research Use Only
RegisterLogin

Anti-CD3 Biotin

CAT: 0270-1B-815-C100Size: 0.1 mgDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0270-1B-815-C100Size:0.1 mg
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
CD3 complex is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules. This association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkynje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases.
Certification
RUO
Reactivity
Human
Target Antigen
CD3
Clone
TB3
Applications
ICC, FC (QC tested)
Concentration
1 mg/mL
Format
Biotin
Buffer
Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
References & Citations
*Khvastunova AN, Kuznetsova SA, Al-Radi LS, Vylegzhanina AV, Zakirova AO, Fedyanina OS, Filatov AV, Vorobjev IA, Ataullakhanov F: Anti-CD antibody microarray for human leukocyte morphology examination allows analyzing rare cell populations and suggesting preliminary diagnosis in leukemia. Sci Rep. 2015 Jul 27;5:12573., URL: https://pubmed.ncbi.nlm.nih.gov/26212756/, *Kužílková D, Puñet-Ortiz J, Aui PM, Fernández J, Fišer K, Engel P, van Zelm MC, Kalina T: Standardization of workflow and flow cytometry panels for quantitative expression profiling of surface antigens on blood leukocyte subsets: An HCDM CDMaps initiative. Front Immunol. 2022 Feb 11:13:827898. , URL: https://pubmed.ncbi.nlm.nih.gov/35222411/
Other References
*Huang Y, Wange RL: T cell receptor signaling: beyond complex complexes. J Biol Chem. 2004 Jul 9;279 (28) :28827-30., URL: https://pubmed.ncbi.nlm.nih.gov/15084594/, *Alarcón B, Swamy M, van Santen HM, Schamel WW: T-cell antigen-receptor stoichiometry: pre-clustering for sensitivity. EMBO Rep. 2006 May;7 (5) :490-5., URL: https://pubmed.ncbi.nlm.nih.gov/16670682/, *Kuhns MS, Davis MM, Garcia KC: Deconstructing the form and function of the TCR/CD3 complex. Immunity. 2006 Feb;24 (2) :133-9., URL: https://pubmed.ncbi.nlm.nih.gov/16473826/
Storage Conditions
Store at 2-8°C. Do not freeze.
Specificity
The mouse monoclonal antibody TB3 recognizes an extracellular epitope on CD3 antigen of the TCR/CD3 complex on mature human T cells. This antibody has superior binding than the clone TB2.
Applications Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml.
Isotype
Mouse IgG2b kappa

Related Products

CatalogName
1B-514-C025Anti-Hu CD3 Biotin
1B-202-C025Anti-Hu CD3 Biotin
1B-202-C100Anti-Hu CD3 Biotin
1B-514-C100Anti-Hu CD3 Biotin
1B-578-C100Anti-Ms CD3 Biotin
1P-578-C100Anti-Ms CD3 PE
1A-202-T100Anti-Hu CD3 APC
11-173-C100Anti-Hu CD3 Purified
PC-514-T100Anti-Hu CD3 PerCP
PC-202-T025Anti-Hu CD3 PerCP

Alternative Products

CatalogName
MBS6121149-01CD3 (CD3) (Biotin)
MBS696604-01Anti-Human CD3 Biotin
MBS666729-01anti-human CD3-Biotin
MBS1801246-01Anti-Hu CD3 Biotin
GT06719Biotin anti-human CD3
E16FMB003-500UBiotin anti-mouse CD3
GT00075Biotin anti-mouse CD3ε
MBS666009-01anti-mouse CD3-Biotin
MBS1800042-01Anti-Ms CD3 Biotin
030846-BiotinCD3 (CD3) (Biotin) discontinued

Popular Products