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Human PSMb1 (Proteasome Subunit Beta Type 1) Microsample ELISA Kit

CAT: 0855-ELK8540MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8540MS-01Size:48 Tests
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Product Name Alternative
PSC5, HC5, Macropain subunit C5; PSMb1
UniProt
P20618
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human proteasome. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human proteasome. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human proteasome, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human proteasome in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.126 ng/mL

UniProtKB · P20618

Proteasome subunit beta type-1

PSB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P20618
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PSMB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B5BU76, Q9BWA8
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinPhosphoprotein

Cellular component

CytoplasmNucleusProteasome

Disease

DeafnessIntellectual disability

Biological process

Host-virus interaction