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Human PSMa5 (Proteasome Subunit Alpha Type 5) Microsample ELISA Kit

CAT: 0855-ELK4543MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4543MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
PSC5; Macropain zeta chain; Multicatalytic endopeptidase complex zeta chain; Proteasome zeta chain
UniProt
P28066
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMa5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMa5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMa5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMa5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.121 ng/mL

UniProtKB · P28066

Proteasome subunit alpha type-5

PSA5_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28066
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PSMA5
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
B2R8F6, B4E2V4, Q3T1C1, Q6IBF7
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationGlycoproteinPhosphoprotein

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmNucleusProteasome

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