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Human PSMb6 (Proteasome Subunit Beta Type 6) Microsample ELISA Kit

CAT: 0013-GTR18872565-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872565-01Size:48 Tests
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Description
Human PSMb6 (Proteasome Subunit Beta Type 6) Microsample ELISA Kit
Product Name Alternative
LMPY; Macropain delta chain; Proteasome subunit Y; Multicatalytic endopeptidase complex delta chain
UniProt
P28072
Reactivity
Human
Field of Research
Protein Biochemistry
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Sensitivity
0.141 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human PSMb6. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human PSMb6. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human PSMb6, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human PSMb6 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.32-20 ng/mL

UniProtKB · P28072

Proteasome subunit beta type-6

PSB6_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P28072
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
PSMB6
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.25.1
Processing
Precursor
Secondary accessions
Q96J55
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinZymogen

Cellular component

CytoplasmNucleusProteasome

Biological process

Host-virus interaction

Molecular function

HydrolaseProteaseThreonine protease

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