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Rat CUBN (Cubilin) Microsample ELISA Kit

CAT: 0855-ELK7401MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7401MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
IFCR; MGA1; gp280; Intrinsic Factor Cobalamin Receptor; 460 kDa receptor; Intestinal intrinsic factor receptor; Intrinsic factor-vitamin B12 receptor
UniProt
O70244
Reactivity
Rat
Field of Research
Signal transduction; Metabolic pathway; Nutrition metabolism; Dermatology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CUBN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CUBN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CUBN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CUBN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.11 ng/mL

UniProtKB · O70244

Cubilin

CUBN_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
O70244
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Cubn
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

Ligand

CalciumCobalaminCobaltMetal-binding

Cellular component

Cell membraneEndosomeLysosomeMembrane

Biological process

Cholesterol metabolismLipid metabolismProtein transportSteroid metabolismSterol metabolismTransport

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoproteinPhosphoprotein

Technical term

Direct protein sequencingReference proteome

Domain

EGF-like domainRepeatSignal

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