Rat NUCB2 (Nucleobindin 2) Microsample ELISA Kit

CAT: 0855-ELK7022MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK7022MS-01Size:48 Tests

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Product Name Alternative

NUCB2; NEFA; Nucleobindin 2; DNA-binding protein NEFA; Gastric cancer antigen Zg4; Prepronesfatin

UniProt

Q9JI85

Reactivity

Rat

Field of Research

Signal transduction

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat NUCB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat NUCB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat NUCB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat NUCB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

50 ng/mL

Sample Type

Serum, plasma and other biological fluids.

Detection Range

0.78-50 ng/mL

Sensitivity

0.34 ng/mL

UniProtKB · Q9JI85

Nucleobindin-2

NUCB2_RAT · Rattus norvegicus

View on UniProt ↗

Primary accession: Q9JI85
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Nucb2
Protein existence: 1: Evidence at protein level
Organism: Rattus norvegicus (Rat)
Taxonomy ID: 10116
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Ligand

CalciumMetal-binding

PTM

Cleavage on pair of basic residuesPhosphoprotein

Cellular component

CytoplasmEndoplasmic reticulumGolgi apparatusMembraneNucleusSecreted

Molecular function

DNA-bindingGuanine-nucleotide releasing factor

Technical term

Reference proteome

Domain

RepeatSignal

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