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Mouse NUCB1 (Nucleobindin 1) Microsample ELISA Kit

CAT: 0855-ELK6865MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6865MS-01Size:48 Tests
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Product Name Alternative
NUC; CALNUC
UniProt
Q02819
Reactivity
Mouse
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NUCB1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NUCB1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NUCB1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NUCB1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.063 ng/mL

UniProtKB · Q02819

Nucleobindin-1

NUCB1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q02819
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Nucb1
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q3TU42, Q9CWA1, Q9D8J4, Q9DBL3
Protein keywords

Ligand

CalciumMetal-binding

Domain

Coiled coilRepeatSignal

Cellular component

CytoplasmGolgi apparatusMembraneSecreted

Technical term

Direct protein sequencingReference proteome

Molecular function

DNA-bindingGuanine-nucleotide releasing factor

PTM

Phosphoprotein

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