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Mouse GDF3 (Growth Differentiation Factor 3) Microsample ELISA Kit

CAT: 0855-ELK6281MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6281MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Vgr2; Vg-Related Gene 2
UniProt
Q07104
Reactivity
Mouse
Field of Research
Cytokine; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDF3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
7.82-500 pg/mL
Sensitivity
2.9 pg/mL

UniProtKB · Q07104

Growth/differentiation factor 3

GDF3_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q07104
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gdf3
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
GDF-3
EC number
—
Processing
Precursor
Secondary accessions
Q3TUX1
Protein keywords

Molecular function

CytokineDevelopmental proteinGrowth factor

Cellular component

CytoplasmSecreted

PTM

Disulfide bondGlycoprotein

Technical term

Reference proteome

Domain

Signal

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