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Mouse GDF5 (Growth Differentiation Factor 5) Microsample ELISA Kit

CAT: 0855-ELK6710MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6710MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CDMP1; LAP4; SYNS2; BMP14; Radotermin; Cartilage-Derived Morphogenetic Protein-1; Bone morphogenetic protein 14; Lipopolysaccharide-associated protein 4
UniProt
P43027
Reactivity
Mouse
Field of Research
Cytokine; Signal transduction; Neuro science; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GDF5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GDF5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GDF5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GDF5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.16-10 ng/mL
Sensitivity
0.064 ng/mL

UniProtKB · P43027

Growth/differentiation factor 5

GDF5_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P43027
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gdf5
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
GDF-5
EC number
—
Processing
Precursor
Secondary accessions
A2ARK2
Protein keywords

Cellular component

Cell membraneMembraneSecreted

Biological process

Chondrogenesis

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineGrowth factor

Technical term

Reference proteome

Domain

Signal

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