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Human GDF3 (Growth Differentiation Factor 3) Microsample ELISA Kit

CAT: 0855-ELK4205MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4205MS-01Size:48 Tests
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Product Name Alternative
Vgr2; Vg-Related Gene 2
UniProt
Q9NR23
Reactivity
Human
Field of Research
Cytokine; Developmental science; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human GDF3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human GDF3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human GDF3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human GDF3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
29 pg/mL

UniProtKB · Q9NR23

Growth/differentiation factor 3

GDF3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9NR23
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
GDF3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
GDF-3
EC number
—
Processing
Precursor
Secondary accessions
Q8NEJ4
Protein keywords

PTM

Cleavage on pair of basic residuesDisulfide bondGlycoprotein

Molecular function

CytokineDevelopmental proteinGrowth factor

Cellular component

CytoplasmSecreted

Disease

Disease variantMicrophthalmia

Technical term

Proteomics identificationReference proteome

Domain

Signal

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