Mouse NPNT (Nephronectin) Microsample ELISA Kit

CAT: 0855-ELK6162MS-02Size: 96 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK6162MS-02Size:96 Tests

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Product Name Alternative

EGFL6L; POEM; Preosteoblast EGF-like repeat protein with MAM domain; Protein EGFL6-like

UniProt

Q91V88

Reactivity

Mouse

Field of Research

Kidney biomarker

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse NPNT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse NPNT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse NPNT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse NPNT in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

10 ng/mL

Sample Type

Tissue homogenates, cell lysates and other biological fluids

Detection Range

0.16-10 ng/mL

Sensitivity

0.06 ng/mL

UniProtKB · Q91V88

Nephronectin

NPNT_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: Q91V88
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Npnt
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: Q6NV58, Q80VP6, Q91XL5, Q91ZD3, Q923T5

Protein keywords

Coding sequence diversity

Alternative splicing

Ligand

Calcium

Biological process

Cell adhesionDifferentiation

Molecular function

Developmental protein

PTM

Disulfide bond

Domain

EGF-like domainRepeatSignal

Cellular component

Extracellular matrixSecreted

Technical term

Reference proteome

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