Mouse MSLN (Mesothelin) Microsample ELISA Kit

CAT: 0855-ELK6254MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK6254MS-01Size:48 Tests
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AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
SMR; MPF; CAK1; Pre-pro-megakaryocyte-potentiating factor; Megakaryocyte-potentiating factor
UniProt
Q61468
Reactivity
Mouse
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse MSLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse MSLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse MSLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse MSLN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.06 ng/mL

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