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Rat VNN1 (Vanin 1) Microsample ELISA Kit

CAT: 0855-ELK6147MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6147MS-01Size:48 Tests
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Product Name Alternative
Pantetheinase; Pantetheine hydrolase; Tiff66; Vascular non-inflammatory molecule 1
UniProt
Q4KLZ0
Reactivity
Rat
Field of Research
Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat VNN1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat VNN1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat VNN1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat VNN1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, urine, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.058 ng/mL

UniProtKB · Q4KLZ0

Pantetheinase

Q4KLZ0_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q4KLZ0
Review status
UniProtKB unreviewed (TrEMBL)
Gene
Vnn1
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.5.1.92
Processing
—
Secondary accessions
—
Protein keywords

Cellular component

Cell membraneMembrane

PTM

GlycoproteinGPI-anchorLipoprotein

Molecular function

Hydrolase

Technical term

Proteomics identificationReference proteome

Domain

Signal

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