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Human BMPR2 (Bone Morphogenetic Protein Receptor 2) Microsample ELISA Kit

CAT: 0855-ELK5158MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5158MS-01Size:48 Tests
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Product Name Alternative
BMPRII; BMPR-II; BRK3; T-ALK; BMPR3; PPH1; Primary Pulmonary Hypertension 1; BMP type II receptor
UniProt
Q13873
Reactivity
Human
Field of Research
Signal transduction; CD & Adhesion molecule; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BMPR2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BMPR2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BMPR2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BMPR2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.123 ng/mL

UniProtKB · Q13873

Bone morphogenetic protein receptor type-2

BMPR2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13873
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BMPR2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
BMP type-2 receptor, BMPR-2
EC number
2.7.11.30
Processing
Precursor
Secondary accessions
Q13161, Q16569, Q4ZG08, Q53SA5, Q585T8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Ligand

ATP-bindingMagnesiumManganeseMetal-bindingNucleotide-binding

Cellular component

Cell membraneMembrane

Disease

Disease variant

PTM

Disulfide bondGlycoproteinPhosphoprotein

Molecular function

KinaseReceptorSerine/threonine-protein kinaseTransferase

Domain

SignalTransmembraneTransmembrane helix

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