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Human LMF2 (Lipase Maturation Factor 2) Microsample ELISA Kit

CAT: 0855-ELK5126MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5126MS-01Size:48 Tests
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Product Name Alternative
TMEM153; TMEM112B; Transmembrane Protein 153
UniProt
Q9BU23
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LMF2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LMF2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LMF2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LMF2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.06 ng/mL

UniProtKB · Q9BU23

Lipase maturation factor 2

LMF2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q9BU23
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LMF2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A6NEZ0, Q13392, Q6ZNR2, Q8WU74, Q96C62
Protein keywords

Coding sequence diversity

Alternative splicing

Cellular component

Endoplasmic reticulumMembrane

PTM

Glycoprotein

Technical term

Proteomics identificationReference proteome

Domain

TransmembraneTransmembrane helix

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