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Human LIPH (Lipase H) Microsample ELISA Kit

CAT: 0855-ELK4100MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4100MS-01Size:48 Tests
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Product Name Alternative
LPDLR; MPAPLA1; PLA1B; mPA-PLA1; LPD lipase-related protein; Phospholipase A1 member B; Membrane-associated phosphatidic acid-selective phospholipase A1-alpha
UniProt
Q8WWY8
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LIPH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LIPH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LIPH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LIPH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
Detection Range
1.57-100 ng/mL
Sensitivity
0.67 ng/mL

UniProtKB · Q8WWY8

Lipase member H

LIPH_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q8WWY8
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LIPH
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
LIPH
EC number
3.1.1.-
Processing
Precursor
Secondary accessions
A2IBA7, Q8TEC7
Protein keywords

Cellular component

Cell membraneMembraneSecreted

Disease

Disease variantHypotrichosis

PTM

Disulfide bondGlycoprotein

Molecular function

Heparin-bindingHydrolase

Biological process

Lipid degradationLipid metabolism

Technical term

Proteomics identificationReference proteome

Domain

Signal

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