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Mouse APOA5 (Apolipoprotein A5) Microsample ELISA Kit

CAT: 0855-ELK4638MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4638MS-01Size:48 Tests
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Product Name Alternative
Apo-A5; APOA-V; APOAV; RAP3; Regeneration-associated protein 3
UniProt
Q8C7G5
Reactivity
Mouse
Field of Research
Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOA5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOA5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOA5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOA5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.129 ng/mL

UniProtKB · Q8C7G5

Apolipoprotein A-V

APOA5_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q8C7G5
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Apoa5
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
Apo-AV, ApoA-V
EC number
—
Processing
Precursor
Secondary accessions
Q91X90, Q99P64
Protein keywords

Cellular component

ChylomicronEndosomeGolgi apparatusHDLSecretedVLDL

Domain

Coiled coilSignal

Biological process

Lipid transportTransport

PTM

Phosphoprotein

Technical term

Reference proteome