Mouse APOA2 (Apolipoprotein A2) Microsample ELISA Kit

CAT: 0855-ELK10467MS-01Size: 48 TestsDry Ice: NoHazardous: No

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CAT#:0855-ELK10467MS-01Size:48 Tests

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Product Name Alternative

Apo-A2; Apolipoprotein A-II; ProapoA-II; Truncated apolipoprotein A-II

UniProt

P09813

Reactivity

Mouse

Field of Research

Metabolic pathway; Endocrinology; Cardiovascular biology; Hepatology; Nutrition metabolism

Assay Type

Sandwich

Assay Principle

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse APOA2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APOA2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse APOA2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APOA2 in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

3.5h

Standard

100 μg/mL

Sample Type

Serum, plasma and other biological fluids.

Detection Range

1.56-100 μg/mL

Sensitivity

0.55 μg/mL

UniProtKB · P09813

Apolipoprotein A-II

APOA2_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: P09813
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Apoa2
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: Apo-AII, ApoA-II
EC number: —
Processing: Precursor
Secondary accessions: Q3UKX6, Q61317

Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Cellular component

AmyloidHDLSecreted

PTM

Cleavage on pair of basic residuesOxidation

Disease

Disease variant

Biological process

Lipid transportTransport

Domain

Signal

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