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Human COL5a3 (Collagen Type V Alpha 3) Microsample ELISA Kit

CAT: 0013-GTR18873621-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18873621-01Size:48 Tests
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Description
Human COL5a3 (Collagen Type V Alpha 3) Microsample ELISA Kit
Product Name Alternative
Collagen alpha-3 (V) chain
UniProt
P25940
Reactivity
Human
Field of Research
Musculoskeletal & Connective Tissue Research
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
0.055 ng/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5a3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5a3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL5a3, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL5a3 in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
0.16-10 ng/mL

UniProtKB · P25940

Collagen alpha-3(V) chain

CO5A3_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P25940
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
COL5A3
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q9NZQ6
Protein keywords

Domain

CollagenRepeatSignal

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinHydroxylationProteoglycan

Cellular component

Extracellular matrixSecreted

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