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Human COL5a2 (Collagen Type V Alpha 2) Microsample ELISA Kit

CAT: 0855-ELK3965MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3965MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
COL5-A2; Collagen Alpha-2 (V) chain; AB Collagen
UniProt
P05997
Reactivity
Human
Field of Research
Metabolic pathway; Developmental science; Nutrition metabolism; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5a2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5a2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL5a2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL5a2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.14 ng/mL

UniProtKB · P05997

Collagen alpha-2(V) chain

CO5A2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P05997
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
COL5A2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
P78440, Q13908, Q53WR4, Q59GR4, Q6LDJ5, Q7KZ55, Q86XF6, Q96QB0, Q96QB3
Protein keywords

Ligand

CalciumMetal-binding

Domain

CollagenRepeatSignal

Technical term

Direct protein sequencingProteomics identificationReference proteome

Disease

Disease variantEhlers-Danlos syndrome

PTM

Disulfide bondGlycoproteinHydroxylation

Cellular component

Extracellular matrixSecreted

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