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Human COL5 (Collagen Type V) Microsample ELISA Kit

CAT: 0855-ELK8934MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8934MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
Collagen Type V
Reactivity
Human
Field of Research
Metabolic pathway; Developmental science; Nutrition metabolism; Bone metabolism
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human COL5. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human COL5. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human COL5, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human COL5 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
1.087 ng/mL

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