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Human CTSD (Cathepsin D) Microsample ELISA Kit

CAT: 0855-ELK3301MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3301MS-01Size:48 Tests
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Product Name Alternative
CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis, Neuronal 10
UniProt
P07339
Reactivity
Human
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTSD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTSD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTSD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTSD in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.054 ng/mL

UniProtKB · P07339

Cathepsin D

CATD_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P07339
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CTSD
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.23.5
Processing
Precursor
Secondary accessions
Q6IB57
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Alzheimer diseaseDisease variantNeurodegenerationNeuronal ceroid lipofuscinosis

Molecular function

Aspartyl proteaseHydrolaseProtease

PTM

Disulfide bondGlycoproteinZymogen

Cellular component

LysosomeSecreted

Domain

Signal

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