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Rat CTSD (Cathepsin D) Microsample ELISA Kit

CAT: 0013-GTR18872280-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0013-GTR18872280-01Size:48 Tests
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Description
Rat CTSD (Cathepsin D) Microsample ELISA Kit
Product Name Alternative
CPSD; CLN10; Lysosomal Aspartyl Protease; Ceroid-Lipofuscinosis, Neuronal 10
UniProt
P24268
Reactivity
Rat
Field of Research
Neuroscience
Assay Type
Sandwich
Assay Performance Time
3.5h
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Sensitivity
29 pg/mL
Notes
For research use only.
Applications Notes
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat CTSD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat CTSD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat CTSD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat CTSD in the samples is then determined by comparing the OD of the samples to the standard curve.
Dynamic Range
62.5-4000 pg/mL

UniProtKB · P24268

Cathepsin D

CATD_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P24268
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ctsd
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.4.23.5
Processing
Precursor
Secondary accessions
—
Protein keywords

Technical term

3D-structureDirect protein sequencingReference proteome

Molecular function

Aspartyl proteaseHydrolaseProtease

PTM

Disulfide bondGlycoproteinZymogen

Cellular component

LysosomeSecreted

Domain

Signal

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