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Human CTSB (Cathepsin B) Microsample ELISA Kit

CAT: 0855-ELK3184MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3184MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CTS-B; APPS; CPSB; APP secretase
UniProt
P07858
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTSB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTSB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTSB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTSB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.13 ng/mL

UniProtKB · P07858

Cathepsin B

CATB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P07858
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CTSB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.22.1
Processing
Precursor
Secondary accessions
B3KQR5, B3KRR5, Q503A6, Q96D87
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

PTM

AcetylationDisulfide bondGlycoproteinZymogen

Cellular component

Cell membraneLysosomeMembraneSecreted

Molecular function

HydrolaseProteaseThiol protease

Domain

Signal

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