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Mouse CTSB (Cathepsin B) Microsample ELISA Kit

CAT: 0855-ELK7100MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7100MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
CTS-B; APPS; CPSB; APP secretase
UniProt
P10605
Reactivity
Mouse
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse CTSB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse CTSB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse CTSB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse CTSB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
2000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
31.25-2000 pg/mL
Sensitivity
12.6 pg/mL

UniProtKB · P10605

Cathepsin B

CATB_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P10605
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Ctsb
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.22.1
Processing
Precursor
Secondary accessions
Q3UDW7
Protein keywords

Technical term

3D-structureReference proteome

PTM

AcetylationDisulfide bondGlycoproteinZymogen

Cellular component

Cell membraneLysosomeMembraneSecreted

Molecular function

HydrolaseProteaseThiol protease

Domain

Signal

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