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Mouse F7 (Coagulation Factor VII) Microsample ELISA Kit

CAT: 0855-ELK10563MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10563MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
FVII; SPCA; proconvertin; Cothromboplastin; Serum Prothrombin Conversation Accelerator; Stable Factor; Eptacog alfa
UniProt
P70375
Reactivity
Mouse
Field of Research
Metabolic pathway; Infection immunity; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F7. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F7, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F7 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
1000 ng/mL
Sample Type
Plasma
Detection Range
15.63-1000 ng/mL
Sensitivity
0.3 ng/mL

UniProtKB · P70375

Coagulation factor VII

FA7_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P70375
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
F7
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.4.21.21
Processing
Precursor
Secondary accessions
Q61109
Protein keywords

Technical term

3D-structureReference proteome

Biological process

Blood coagulationHemostasis

Ligand

Calcium

PTM

Cleavage on pair of basic residuesDisulfide bondGamma-carboxyglutamic acidGlycoproteinHydroxylationZymogen

Domain

EGF-like domainRepeatSignal

Molecular function

HydrolaseProteaseSerine protease

Cellular component

Secreted

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