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Mouse F8 (Coagulation Factor VIII) Microsample ELISA Kit

CAT: 0855-ELK10479MS-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10479MS-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Product Name Alternative
FVIII; AHF; HEMA; F8B; F8C; Antihemophilic Globulin; Hemophilia A; Anti-Hemophilic Factor A; FVIII Procoagulant Component; F8 Protein
UniProt
Q06194
Reactivity
Mouse
Field of Research
Metabolic pathway; Hematology
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse F8. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse F8. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse F8, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse F8 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 IU/mL
Sample Type
Plasma
Detection Range
0.32-20 IU/mL
Sensitivity
0.081 IU/mL

UniProtKB · Q06194

Coagulation factor VIII

FA8_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
Q06194
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
F8
Protein existence
2: Evidence at transcript level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A2AN88
Protein keywords

Biological process

Acute phaseBlood coagulationHemostasis

Ligand

CalciumMetal-binding

PTM

Disulfide bondGlycoproteinSulfation

Technical term

Reference proteome

Domain

RepeatSignal

Cellular component

Secreted

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