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Human LTF (Lactoferrin) ELISA Kit

CAT: 0855-ELK10188-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK10188-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
UniProt
P02788
Reactivity
Human
Field of Research
Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Detection Range
78.13-5000 ng/mL
Sensitivity
21.3 ng/mL

UniProtKB · P02788

Lactotransferrin

TRFL_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P02788
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
LTF
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
Lactoferrin
EC number
3.4.21.-
Processing
Precursor
Secondary accessions
A8K9U8, B2MV13, B7Z4X2, E7EQH5, O00756, Q16780, Q16785, Q16786, Q16789, Q5DSM0, Q8IU92, Q8IZH6, Q8TCD2, Q96KZ4, Q96KZ5, Q9H1Z3, Q9UCY5
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative promoter usage

Molecular function

AntibioticAntimicrobialDNA-bindingHeparin-bindingHydrolaseProteaseSerine protease

Cellular component

CytoplasmNucleusSecreted

PTM

Disulfide bondGlycoproteinIsopeptide bondUbl conjugation

Biological process

ImmunityIon transportIron transportOsteogenesisTranscriptionTranscription regulationTransport

Ligand

IronMetal-binding

Domain

RepeatSignal

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