Human LTF (Lactoferrin) Microsample ELISA Kit

CAT: 0855-ELK10188MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK10188MS-01Size:48 Tests
Selected
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Product Name Alternative
HLF2; LF; GIG12; Lactotransferrin; Growth-inhibiting protein 12; Talalactoferrin; Lfcin-H; Kaliocin-1
UniProt
P02788
Reactivity
Human
Field of Research
Tumor immunity; Infection immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human LTF. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human LTF. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human LTF, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human LTF in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 ng/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, breast milk, cell culture supernates and other biological fluids
Detection Range
78.13-5000 ng/mL
Sensitivity
21.3 ng/mL

Popular Products