Rabbit CFD (Complement Factor D) ELISA Kit

CAT: 0855-ELK10153-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK10153-01Size:48 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit CFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit CFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CFD in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CF-D; DF; PFD; ADN; Adipsin; Properdin Factor D; C3 convertase activator
Reactivity
Rabbit
Field of Research
Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rabbit CFD. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rabbit CFD. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rabbit CFD, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rabbit CFD in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 μg/mL
Sample Type
Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
0.16-10 μg/mL
Sensitivity
0.061 μg/mL

Related Products

CatalogName
ELK10499-01Mouse CFD (Complement Factor D) ELISA Kit
ELK10797-01Rat CFD (Complement Factor D) ELISA Kit
ELK1128-01Human CFD (Complement Factor D) ELISA Kit
ELK10499MS-01Mouse CFD (Complement Factor D) Microsample ELISA Kit
ELK3011-01Human CFP (Complement Factor P) ELISA Kit
ELK11088-01Human CFH (Complement Factor H) ELISA Kit
ELK10688-01Rat CFB (Complement Factor B) ELISA Kit
ELK1721ELISA kit for Rat CFH (Complement Factor H)
ELK10320-01Mouse CFH (Complement Factor H) ELISA Kit
ELK11103-01Human CFB (Complement Factor B) ELISA Kit

Alternative Products

CatalogName
MBS727195-01Rabbit Complement Factor P ELISA Kit
MBS011064Rabbit Complement Factor I ELISA Kit
MBS721653-01Rabbit Complement Factor B ELISA Kit
MBS720753-01Rabbit Complement Factor H ELISA Kit
GTR10478173-01Rabbit Complement Factor D ELISA Kit
MBS8821500-01Rabbit CFD (Complement Factor D) ELISA Kit
MBS2504709-01Rabbit CFH (Complement Factor H) ELISA Kit
MBS2515506-01Rabbit CFB (Complement Factor B) ELISA Kit
MBS2601184-01Rabbit Complement Factor H (CFH) ELISA Kit
MBS2806164-01Rabbit Complement factor B (CFB) ELISA Kit

Popular Products