Human CFI (Complement Factor I) ELISA Kit

CAT: 0855-ELK2800-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK2800-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFI in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CF-I; C3b-INA; FI; IF; KAF; Factor I; Konglutinogen-Activating Factor; C3b-Inactivator; C3B/C4B inactivator
UniProt
P05156
Reactivity
Human
Field of Research
Metabolic pathway; Immune molecule; Hematology; Autoimmunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFI in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.072 ng/mL

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