RegisterLogin

Human CFH (Complement Factor H) ELISA Kit

CAT: 0855-ELK11088-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK11088-01Size:48 Tests
Selected
PRICE
AVAILABILITY: InStock
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Product image 1
1 / 1
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFH in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CF-H; FH; FHL1; ARMD4; ARMS1; CFHL3; HF1; HF2; HUS; H Factor 2; Age-Related Maculopathy Susceptibility 1; Adrenomedullin binding protein
UniProt
P08603
Reactivity
Human
Field of Research
Metabolic pathway; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CFH. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CFH. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CFH, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CFH in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 μg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.16-10 μg/mL
Sensitivity
0.048 μg/mL

Related Products

CatalogName
ELK1721ELISA kit for Rat CFH (Complement Factor H)
ELK10320-01Mouse CFH (Complement Factor H) ELISA Kit
ELK10837-01Rat CFH (Complement Factor H) ELISA Kit
ELK10320MS-01Mouse CFH (Complement Factor H) Microsample ELISA Kit
ELK10837MS-01Rat CFH (Complement Factor H) Microsample ELISA Kit
ELK10800-01Human CFHR5 (Complement Factor H Related Protein 5) ELISA Kit
ELK7134-01Human CFHR1 (Complement Factor H Related Protein 1) ELISA Kit
ELK6306-01Human CFHR3 (Complement Factor H Related Protein 3) ELISA Kit
ELK3011-01Human CFP (Complement Factor P) ELISA Kit
ELK2800-01Human CFI (Complement Factor I) ELISA Kit

Alternative Products

CatalogName
AP-E100142-01Human Complement Factor B ELISA Kit
PC088Human Complement Factor D ELISA Kit
MBS025377-01Human Complement Factor I ELISA Kit
MBS9425913-01Human Complement factor H ELISA Kit
MBS024173-01Human Complement Factor P ELISA Kit
HK342-01Complement factor H, Human, ELISA kit
HK343-01Complement Factor D, Human, ELISA kit

Popular Products